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cholinergic receptor, nicotinic, beta 2 (CHRNB2, Acrb2, N-alpha 1, Neuronal acetylcholine receptor non-alpha-1 chain, Neuronal acetylcholine receptor subunit beta-2)

cholinergic receptor, nicotinic, beta 2 (CHRNB2, Acrb2, N-alpha 1, Neuronal acetylcholine receptor non-alpha-1 chain, Neuronal acetylcholine receptor subunit beta-2)

Cat no: C5062-74X

Supplier: United States Biological
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Nicotinic acetylcholine receptors, also known as nAChRs, belong to a family of proteins called ligand gated ion channels and are widely expressed throughout the nervous system. nAChRs are made up of 5 subunits (two alpha and three beta). Subunits a4 and b2 (a4b2) is the main receptor in the mammalian brain and has been associated with the positive reinforcing effects of nicotine. The relative contribution of nAChRs b2 and b4 are not well understood because the subunit structure that mediates synaptic transmission in the peripheral nervous system is unknown. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:20000 Optimal dilutions to be determined by the researcher. Positive Control: Rat e16 brain lysate Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C5062-74X
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Western Blot
Size: 100ug
Form: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
References: 1. Brody, A., et al. (2006). Arch Gen Psychiatry. 63:907-915.
Additional info: Recognizes rat CHRNB2. Species Crossreactivity: human, mouse.

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