Chondroitin Sulfate, Proteoglycan Mixture (CSPG)

100ug protein as determined using the bicinchoninic acid (BCA) assay. Note that 100ug is a measurement of core polypeptides. Components of this mixture contain core polypeptide plus attached glycosaminoglycans. Contains a mixture of large, extracellular chondroitin sulfate proteoglycans isolated from embryonic chicken brain. Amino acid sequence analyses and immunological studies have demonstrated that major components of mixture are neurocan, phosphacan, versican and aggrecan.\n\nAggrecan, versican and neurocan are members of the family of hyaluronic acid-binding proteoglycans and therefore each contains hyaluronic acid binding regions, Ig-like domains, EGF like domains, lectin-like domains, and complement regulatory-like domains. Phosphacan is distinct; contains a region of similarity to carbonic anhydrase, a fibronectin type III domain, and is a splice variant of transmembrane protein receptor tyrosine phosphatase equivalent to its extracellular portion.\n\nAlthough aggrecan was traditionally thought of as cartilage proteoglycan and neurocan and phosphacan were thought of as neural proteoglycans, recent studies indicate that all four proteoglycans have broad yet distinctive distribution patterns in early embryos. Presence of these proteoglycans has not been carefully examined in adult tissue beyond the expected sites. These proteoglycans are anti-adhesion molecules and capable of inhibiting cell-ECM and cell-cell adhesion.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C5067-70C

Size

100ug

Form

Supplied as a liquid in PBS. No preservatives added.

Purity

~95% by PAGE analysis; Migrates as a large HMW (< 400kD) mass under reduced conditions. Mixture of large, extracellular chondroitin sulfate proteoglycans purified from embryonic chicken brains by chromatrographic methods. Following chondroitinase treatment to remove the glycosaminoglycan portion of these proteoglycans, the major components of this preparation resolved on SDS gels are 250kD core proteins representing neurocan and 400kD core proteins representing aggrecan, phosphacan and versican.

References

1. Ernst, H., et al., J. Cell Sci. 108: 3807-3816 (1995). 2. Li, H., et al., J. Biol. Chem. 268: 23504-23511 (1995). 3. Shinomura, T., et al., J. Biol. Chem. 268: 14461-14469 (1993). 4. Rauch, U., et al., J. Biol. Chem. 267: 19536-19547 (1992). 5. Maurel, P., et al., Proc. Natl. Acad. Sci. USA 91: 2512-2516 (1994). 6. Balsamo, J., et al., J. Cell Biol. 129: 1391-1401 (1995).