Chondroitin Sulfate Proteoglycan (NG2, CSPG)

The NG2 chondroitin sulfate proteoglycan is a valuable marker for many types of immature progenitor cells including pericytes/smooth muscle cells, chondroblasts and oligodendrocyte progenitors. NG2 is often expressed in melanomas, chondrosarcomas, glioblastomas and myeloid leukemias. Studies indicate that NG2 is involved in modulating cellular response to growth factors and cell motility; these are critical functions in proliferation and migration of immature progenitor cells and tumor cells. Oligodendrocyte precursor cells (OPCs) are a population of NG2 expressing cells in the brain that accumulate in response to CNS injuries. OPCs, together with microglia and astrocytes, form the glial scar, which is believed to inhibit or prevent axonal regeneration partly by producing growth inhibiting NG2 at the injury site8. It has been suggested that inhibiting NG2 at sites of spinal cord injury may increase beneficial axonal regeneration. Studies suggest that NG2 stimulates tumor growth and neovascularization in glioblastoma multiforme by inhibiting angiostatin and thus promoting angiogenesis. As NG2 is associated with increased tunmor cell proliferation, it may be a potential therapeutic marker in brain tumors.\n\nApplications:\nSuitable for use in Immunofluorescence and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nImmunohistochemistry (Frozen): 1:100-1:400\nImmunofluorescence: 1:100-1:400\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C5067-70M

Size

100ul

Applications

IF, IHC

Hosts

Mouse

Reactivities

Hum, Mouse, Bov, Ch/Bird

Form

Supplied as a liquid, 0.09% sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgM

References

1. Avnur, Z. et al (1985) Spatial interrelationships between proteoglycans and extracellular matrix proteins in cell cultures. Exp Cell Res. 158:321-32. 2. Avnur, Z.et al (1984) Immunocytochemical Localization of Native Chondroitin-Sulphate in Tissues and Cultured Cells Using Specific Monoclonal Antibody. Cell 38: 811-822.

Additional Info

Recognizes chicken Chondroitin sulfate types A and C (not B, dermatan sulfate). Gives positive staining in bovine mammary gland epithelial cells and human, chicken and mouse fibroblast or tissues. Species Crossreactivity: bovine, human and mouse.