Ciliary Neurotrophic Factor (CNTF)

CNTF is a survival promoting factor for different types of neurons in vitro and in vivo. The essential structural features for the biological function of human CNTF were investigated by Thier, M. et al. They showed that deletion of 14 N-terminal and 18 C-terminal amino acids significantly increased bioactivity compared to wild-type CNTF. CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. SUBUNIT: Homodimer. \n\nCellular Localization:\nCytoplasm. \n\nTissue Specificity:\nNervous system. PHARMACEUTICAL: CNTF is being tested under the name Axokine by Regeneron Pharmaceuticals for treatment of human motor neuron diseases, such as amyotrophic lateral sclerosis (ALS). As it induces substantial weight loss, preferentially of fat as opposed to lean body mass, it is being used for obesity treatment. Belongs to the CNTF family.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C5073-09E

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder. Reconstitute in 100ul of sterile water. Centrifuge to remove any insoluble material.

P Type

Pab

Purity

Serum

References

1. LF Lin etal (1989) Science 246, 1023-5 ; 2. KA Stockli etal (1991) J Cell Biol 115, 447-59 ; 3. I Saggio etal (1995) Embo J 14, 3045-54 ; 4. DM Hermann etal (2001) Neurobiol Dis 8, 655-66 ; 5. M Thier etal (1995) J Neurosci Res 40, 826-35

Additional Info

This antibody specifically detects CNTF shown by western blot. Species Crossreactivity: This antiserum to known to react with rat, mouse and human CNTF protein.

Alternative Names

Ciliary neurotrophic factor