Ciliary Neurotrophic Factor, Recombinant, Human (CNTF)

A neurotrophic factor that promotes the survival of various neuronal cell types and may play an important role in the injury response in the nervous system. CNTF, like FGF acidic, FGF basic, and PD-ECGF (platelet-derived endothelial cell growth factor), does not possess a signal sequence that would allow secretion of the factor by classical secretion pathways (endoplasmatic reticulum/Golgi system). The mechanism underlying the release of CNTF is unknown.\n\nRecombinant Human CNTF is a single, non-glycosylated polypeptide chain containing 199 amino acids and having a molecular mass of 22,706 Daltons. \n\nSource:\nE. coli\n\nSequence:\nThe sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Phe-Thr-Glu. \n\nDimers and Aggregates:\n(same/less than) 1% as determined by silver-stained SDS-PAGE gel analysis. \n\nEndotoxin:\n(same/less than) 0.1ng/ug (IEU/ug)\n\nProtein Content:\nProtein quantitation was carried out by two independent methods: \n1. UV spectroscopy at 280nm using the absorbency value of 1.28 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). \n2. Analysis by RP-HPLC, using a standard solution of human CNTF as a Reference Standard. \n\nBiological Activity:\nRecombinant Human Ciliary Neurotrophic Factor is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of TF-1 cells is less then 2 ng/ml, corresponding to a Specific Activity of 5 x 10 IU/mg. \n\nReconstitution:\nRecommended to reconstitute the lyophilized CNTF in sterile 18MOmega-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. \n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C5073-06A

Size

5ug

Form

Supplied as a lyophilized powder in 5mM sodium phosphate buffer, pH 7.5, 5mM sodium chloride.

Purity

(same/more than) 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

1. Setoguchi T, Kondo T, J Cell Biol 2004 Sep 27;166(7):963-8.\n2. McCallister WV, McCallister EL, Trumble TE, J Reconstr Microsurg 2004 Aug;20(6):473-81.\n3. Calcutt NA, Freshwater JD, Diabetologia 2004 Apr;47(4):718-24.\n4. Bongioanni P, Reali C, Cochrane Database Syst Rev 2004;(3):CD004302.\n5. Bush RA, Lei B, Raz D, Sieving PA, Invest Ophthalmol Vis Sci 2004 Jul;45(7):2420-30.\n6. Kimata H, Neuropeptide s 2004 Apr-Jun;38(2-3):92-7.