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Ciliary Neurotrophic Factor Recombinant, Human (CNTF)

Cat no: C5073-06C

Ciliary Neurotrophic Factor Recombinant, Human (CNTF)

CNTF (Ciliary neurotrophic factor) is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. This protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. CNTF protein is structurally related to leukemia inhibitory factor (LIF), interleukin-6 (IL-6), interleukin-11 (IL-11) and oncostatin M (OSM). It is localized in the cell nucleus subsequent to receptor binding. Recombinant human CNTF, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.\n\nMolecular Weight: \n25kD (220 aa)\n\nSequences: \nMGSSHHHHHH SSGLVPRGSH MAFTEHSPLT PHRRDLCSRS IWLARKIRSD LTALTESYVK HQGLNKNINL DSADGMPVAS TDQWSELTEA ERLQENLQAY RTFHVLLARL LEDQQVHFTP TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEAD GMPINVGDGG \nLFEKKLWGLK VLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKM\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C5073-06C

Size

100ug

Applications

WB

Reactivities

Hum

Form

Supplied as a liquid in 20mM Tris pH 8.0, 1mM DTT.

Purity

(same/more than) 95% by SDS PAGE.

References

Sendther M., et al. (1994), J Neurobiol. 25(11):1436-53.\nRobert F., et al. (2007), J. Biol. Chem. 282(46): 33421-33434.

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