Ciliary Neurotrophic Factor, Recombinant, Human (CNTF, HCNTF)

Source:\nRecombinant corresponding to 199aa of human Ciliary Neurotrophic Factor expressed in E.coli is a single, non-glycosylated polypeptide chain.\n\nMolecular Weight:\n~22.7kD\n\nBiological Activity:\nThe ED50 as determined by the dose-dependent stimulation of TF-1 cells is less then 2ng/ml, corresponding to a Specific Activity of 5x10 IU/mg.\n\nDimers and Aggregates:\n(same/less than)1% (SDS-PAGE)\n\nProtein Content:\nProtein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280nm using the absorbency value of 1.28 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of human CNTF as a Reference Standard.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C5073-06J

Size

1 Vial

Form

Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to (same/more than)0.1mg/ml.

Purity

~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: (same/less than)0.1ng/ug (IEU/ug)

References

1. Relation of weight maintenance and dietary restraint to peroxisome proliferator-activated receptor gamma2, glucocorticoid receptor, and ciliary neurotrophic factor polymorphisms. Am J Clin Nutr 2005 Oct;82(4):740-6. 2. Ciliary neurotrophic factor-immunoreactivity in olfactory sensory neurons. Neuroscience 2005;134(4):1179-94. 3. Ciliary neurotrophic factor promotes muller glia differentiation from the postnatal retinal progenitor pool. Dev Neurosci 2004;26(5-6):359-70. 4. Dedifferentiation of adult human myoblasts induced by ciliary neurotrophic factor in vitro. Mol Biol Cell 2005 Jul;16(7):3140-51/ 5. Rapid protein kinase C-dependent reduction of rat skeletal muscle voltage-gated sodium channels by ciliary neurotrophic factor. J Physiol 2005 Jun 15;565(Pt 3):827-41. 6. E xpression of ciliary neurotrophic factor (CNTF), CNTF receptor alpha (CNTFR-alpha) following experimental intracerebral hemorrhage in rats. Neurosci Lett 2005 Apr 4;377(3):170-5.