Citrate BioAssay(TM) Kit

Citric acid (HOOC-CH2-C(-OH)(-COOH) -CH2-COOH) is a key intermediate in the TCA cycle which occurs in mitochondria. It is formed by the addition of oxaloacetate to the acetyl group of acetyl-CoA derived from the glycolytic pathway. Citrate can be transported out of mitochondria and converted back to acetyl CoA for fatty acid synthesis. Citrate is an allosteric modulator of both fatty acid synthesis (acetyl-CoA carboxylase) and glycolysis (phospho-fructokinase). Citrate is widely used industrially in foods, beverages and pharmaceuticals. Citrate metabolism and disposition can vary widely due to sex, age and a variety of other factors. \n\nCitrate BioAssay(TM) Kit provides a simple, sensitive and rapid means of quantifying citrate in a variety of samples. In the assay, citrate is converted to pyruvate via oxaloacetate. The pyruvate is quantified by converting a nearly colorless probe to an intensely colored (lmax W0nm) and fluorescent (Ex/Em, 535/587nm) product. The Citrate BioAssay(TM) Kit can detect 0.1-10nmoles (~2uM-10mM) of citrate in a variety of samples.\n\nKit Components: \nC5802A: Assay Buffer, 1x25ml\nC5802B: Probe, 1x200ul\nC5802D: Enzyme Mix, 1x1 vial\nC5802E: Developer, 1x1 vial\nC5802F: Standard (10umol), 1x1 vial\n\nStorage and Stability:\nStore kit at -20 degrees C. Protect from light. Allow C5802A: Assay Buffer to warm to room temperature before use. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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