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Citrulline

Cat no: 029445

Citrulline

Citrulline is thought to be an unincorporated amino acid that plays a part in intermediary metabolism. It is produced in the small intestine by enterocytes. Citrulline concentration in plasma and serum is thought to be a reliable and noninvasive biomarker of small intestine function and mass during disease. Citrulline has also been shown to play an important role in protein homeostasis. Modified citrulline is a chemically modified form of citrulline that is more immunogenic.\n\nApplications:\nSuitable for use in ELISA, Dot Blot and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nDot Blot: 1:125\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nModified Tape-Stripped Cornified cells\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

029445

Size

100ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

References

1. Oliverius, M., et al. (2010). Cas Lek Cesk. 149(4):160-162. 2. Crenn, P., et al. (2008). Clin Nutr. 27(3):328- 339. 3. Curis, E., et al. (2007). Curr Opin Clin Nutr Metab Care. 10(5):620-626. 4. Moinard, C., et al. (2007). J Nutr. 137(6 Suppl 2):1621S-1625S. 5. Senshu, T., et al. (1995). J Invest Dermatol. 105:163-169. 6. Senshu, T., et al. (1992). Anal Biochem. 203:94-100. 7. Hagiwara, T., et al. (2002). Biochem Biophys Res Commun. 290:979-983. 8. Masson-Bessiere, C., et al. (2001). J Immunol. 166:4177-4184. 9. Ishida-Yamamoto, A., et al. (2000). J Invest Dermatol. 114:701-705.

Additional Info

Recognizes any proteins containing modified citulline, hence various molecular weight bands can be expected.

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