CLAN (NLR Family CARD Domain-containing Protein 4, CARD, LRR, and NACHT-containing Protein, Clan Protein, Caspase Recruitment Domain-containing Protein 12, Ice Protease-activating Factor, Ipaf, NLRC4, CARD12, CLAN1, IPAF, UNQ6189/PRO20215)

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adaptor molecules and proteases including several members of the caspase family. Another family of proteins that functions as a critical regulator of apoptosis and NF-kB signaling pathways is the CED-4/Apaf-1 (apoptosis protein activating factor-1) protein family. Ipaf (ICE protease activating factor) is a CED-4/Apaf-1 family member that activates caspase-1/ICE and can induce apoptosis in human cells in a caspase-1 dependent manner. Ipaf and caspase-1 are thought to interact with each other through the association of the Ipaf amino-terminal CARD (caspase recruitment domain) and amino-terminal CARD of caspase-1.\n\nApplications:\nSuitable for use in Immunofluorescence, Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: 10ug/ml \nWestern Blot: 0.5-2ug/ml \nImmunocytochemistry: 10ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman peripheral blood leukocyte (PBL) cell lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C5820-01E

Size

50ug

Applications

ICC, IF, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Li P, Nijhawan D, Budihardjo I, et al. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997;91:479-89. 2. Poyet J-L, Srinivaula SM, Tnani M, et al. Identification of Ipaf, a human caspase-1-activating protein related to Apaf-1. J. Biol. Chem. 2001;276:28309-13. 3. Geddes BJ, Wang L, Huang WJ, et al. Human CARD12 is a novel CED4/Apaf-1 family member that induces apoptosis. Biochem. Biophys. Res. Commun. 2001;284:77-82.

Additional Info

Recognizes human CLAN.