Clathrin

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network. SUBUNIT: Clathrin triskelions, composed of 3 heavy chains and 3 light chains, are the basic subunits of the clathrin coat. In the presence of light chains, hub assembly is influenced by both the pH and the concentration of calcium. \n\nCellular Localization:\nCytoplasmic vesicle; cytoplasmic vesicle membrane; cytoplasmic side. Cell membrane; coated pit; cytoplasmic side. Melanosome. Note=Cytoplasmic face of coated pits and vesicles. PTM: The N-terminus is blocked. Belongs to the clathrin heavy chain family.\n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C5835-05A

Size

100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a lyophilized powder. Reconstitute in 100ul of sterile water. Centrifuge to remove any insoluble material.

P Type

Pab

Purity

Serum

References

1. Kirchhausen T, et al. Proc. Natl. Acad. Sci. U.S.A. 84:8805-8809(1987). ; 2. Ter Haar E, et al. Cell 95:563-573(1998). ; 3. Chi A, et al. J. Proteome Res. 5:3135-3144(2006). ; 4. Beausoleil S.A, et al. Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004). ; 5. Gevaert K, et al. Nat. Biotechnol. 21:566-569(2003). ; 6. Dodge G.R, et al. Genomics 11:174-178(1991). ; 7. Nomura N, et al. DNA Res. 1:27-35(1994).

Additional Info

This antibody has been shown to be specific for clathrin. Species Crossreactivity: Rat, other species have not yet tested.