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Clathrin Heavy Chain (Clathrin Heavy Chain 1, CLTC Hc, CHC17, Clathrin Heavy Polypeptide, CLH17, CLH-17, CLTCL2, Hc, KIAA0034)

Clathrin Heavy Chain (Clathrin Heavy Chain 1, CLTC Hc, CHC17, Clathrin Heavy Polypeptide, CLH17, CLH-17, CLTCL2, Hc, KIAA0034)

Cat no: C5835-07D

Supplier: United States Biological
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Applications: Suitable for use in ELISA, Immunofluorescence, Immunoprecipitation, and Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C5835-07D
Reactivities: Human, Mouse, Rat, Bovine, Porcine
Hosts: Mouse
Applications: ELISA, Immunofluorescence, Immunoprecipitation, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris, pH 8.0, 0.09% sodium azide.
P type: Mab
Isotype: IgM
Purity: Purified by Ion Exchange chromatography.
References: 1. Macurek, L. et al. (2003) Monoclonal antibody BF-06 against the heavy chain of clathrin. Folia. Biol. (Praha) 49: 238-240.
Additional info: Recognizes mouse clathrin heavy chain (CHC), a ubiquitously expressed protein and major component of clathrin-coated vesicles and pits, which combines with clathrin light chains (CLC) to form clathrin triskelions (3CHC and 3CLC), which then self-assemble into a polyhedral coat. Species Crossreactivity: rat, human, bovine, porcine.

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