Claudin 1 (CLDN1)

The claudin (CLDN) superfamily consists of at least 18 homologous proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport, complexed with two other integral transmembrane proteins, occludin and junctional adhesion molecule. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Claudins interact directly with tight junction-specific, membrane-associated guanylate kinase homologues, ZO-1, ZO-2, and ZO-3, and indirectly with AF-6 and the myosin-binding molecule cingulin. These protein-protein interactions promote scaffolding of the tight junction transmembrane proteins and provide a link to the actin cytoskeleton for transducing regulatory signals to and from tight junctions. Human claudin-1, like other claudin proteins, participates in cell-cell adhesion and regulates paracellular and transcellular transport of solutes across human epithelia and endothelia. Recently, a claudin-1 cDNA has been isolated from human mammary epithelial cells (HMECs). As claudin-1 expression is low or undetectable in a number of breast tumors and breast cancer cell lines, it may be implicated as a possible tumor-suppressor gene.\n\nApplications:\nSuitable for use in ELISA, Western Blot, Immunofluorescence, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilutions:\nELISA: 0.1-1ug/ml\nWestern Blot (human, canine, rat, mouse): 1-3ug/ml\nImmunofluorescence (canine, mouse): 1-5ug/ml\nImmunoprecipitation (human): 10ug/test\nImmunohistochemistry (mouse) 5-10ug/ml on frozen sections.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C5838-02A

Size

100ug

Applications

ELISA, IF, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Can

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1,k

References

1. Heiskala, M., et al., Traffic 2(2): 93-98 (2001). 2. Furuse, M., et al., J. Cell. Biol. 143(2): 391-401 (1998). 3. Kramer, F., et al., Hum. Genet. 107: 249-256 (2000).

Additional Info

Recognizes the C-terminal region of the human claudin-1 protein. On Western Blots, it identifies the target band at ~22kD. Reactivity has been confirmed with human Caco-2 and canine MDCK cell lysates, mouse kidney and intestinal lysates, frozen mouse intestine (jejunum) tissue, and rat kidney homogenates.