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Claudin 18 (CLDN18)

Cat no: C5838-91

Claudin 18 (CLDN18)

Claudin-18 is a tight junction protein expressed as lung- and stomach-specific isoforms which are generated by alternative splicing of the claudin-18 gene.1 The lung-specific form is a downstream target gene regulated by the T/EBP/NKX2.1 transcription factor.1 A splice variant lacking the C-terminal cytoplasmic domain also exists in mouse, but has not been confirmed in human.1 Human claudin-18 demonstrates 88% amino acid sequence identity to the mouse protein.1 Immunohistochemical studies have demonstrated complete membrane localization of claudin-18 in lung and stomach epithelial cells, while electron microscopy has shown that it is concentrated in the cell-cell borders of these cells.1 These features suggest a potentially important role for claudin-18 in the structure and function of tight junctions in the lung and\nstomach.1 Claudin-18 expression has also been reported in the inner ear.2\n\nApplications: \nSuitable for use in Western Blot, Immunofluorescence, and Immunohistochemistry Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-3ug/ml\nImmunohistochemistry (frozen): 5ug/ml\nImmunofluorescence: 5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: Mouse lung and stomach homogenates\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C5838-91

Size

100ug

Applications

IF, IHC, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography

Isotype

IgG

References

1. Niimi T, et al. Mol Cell Biol 21:7380-7390, 2001. 2. Kitajiri SI, et al. Hear Res 187:25-34, 2004.

Additional Info

Recognizes mouse Claudin-18 protein at ~29 kD. \nSpecies sequence homology: Human

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