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Claudin 8 (CLDN8)

Cat no: C5838-56

Claudin 8 (CLDN8)

Claudin-8 is a tight junction protein expressed in several mouse distal nephron segments, suggesting that it may contribute to the paracellular barrier that protects transtubular Na+, K+, and H+ gradients in the distal nephron. Caludin-8 decreases paracellular permeability to cations when overexpressed in MDCK-II cells, suggesting that it may function as a paracellular cation barrier. Caludin-8 is also found in the thin ascending limb of Henle, a segment of the nephron that is known to be leaky to Na+. Claudin-8 is expressed primarily in tight junctions along the entire aldosterone-sensitive distal nephron (ASDN) and in the late thin descending limb of Henle, consistent with its role as a paracellular cation barrier protein. Claudin-8 is found in umbrella cell tight junctions in the bladder epithelium. Expression of Claudin-8 has also been reported in the inner ear.\n\nApplications:\nSuitable for use in Immunohistochemistry, Western Blot and Immunofluorescence. Other applications have not been tested.\n\nRecommended Dilutions:\nImmunohistochemistry (Frozen, mouse): 2.7ug/ml. \nWestern Blot (mouse, rat, canine): 1-3ug/ml. Detects a band at ~30kD.\nImmunofluorscence (mouse): 2.7ug/ml\nOptimal dilutions to be determined by the researcher.\n\nRecommended Controls:\nTCMK-1, KNRK and MDCK cell lysates\n\nRecommended Tissue:\nMouse Colon Tissue\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C5838-56

Size

100ug

Applications

IF, IHC, WB

Hosts

Rabbit

Reactivities

Mouse, Rat, Can

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes Claudin-8. Species Crossreactivity: mouse, rat and canine.

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