CLC7 (Clcn7, Chloride Channel)

Voltage-gated chloride channels regulate cellular traffic of chloride ion. The chloride channels (CIC or CLC) performs several functions including the regulation of cell volume, membrane potential stabilization, signal transduction, and transepithelial transport. Mutations in CIC genes have been linked with several human diseases including myotonias (Thomsen's disease), cystic fibrosis, Bartters syndrome type III, Dent's disease, and X-linked recessive nephrolithiasis. In mammals, CLC proteins form a superfamily of at least 9 different genes (CLC1-7 also known as CLCN1-7 and CLK1-2 or CLCKa and CLCKb). Additional forms of these proteins are obtained by alternative splicing. All CLC proteins (~700-1000 aa) are predicted to contain 10 (possibly 12) transmembrane domains. Except CLC-1 and CLC-K1/K2 that are specific for kidney, most other CLC are widely distributed in various tissues. Rat CLC-7 is 802 aa membrane protein (mouse 803 aa, human 805 aa) (1). CLC-7 has a calculated MW of approx. 90kD. CLC-7 is ~45% identical with CLC-6. CLC-7 is expressed in brain, tested, muscle and kidney (1).\n\nApplications: \nSuitable for use in ELISA, Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-10ug/ml (ECL.) \nELISA: 0.5-1ug/ml Control peptide can be used to coat ELISA plates at 1ug/ml.\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide: \nC5837-56: CLC7, Human (Clcn7, Chloride Channel) \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C5837-59

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4 and 0.1% sodium azide, 40% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

(1). Brandt S et al (1995) FEBS Lett. 377, 15-20; Konark U (1998) Gene accession #AF060399; Eggermont J et al (1998) BBA 1397, 156-160; (2) Kornak U et al (2001) Cell 104, 205-215

Additional Info

Recognizes human CLC7. No significant sequence homology is detected with other CLCs or other proteins. Species sequence homology: mouse and rat 93%.