CLEC9A, Recombinant, Human (C-type Lectin Domain Family 9 Member A, UNQ9341/PRO34046)

CLEC9a (C-type lectin domain family 9 member A), also known as DNGR-1, is a type II transmembrane glycoprotein member of the C-type lectin superfamily. Mature human CLEC9a consists of a 35aa cytoplasmic domain with an ITAM-like motif, a 21aa transmembrane segment, and a 185aa extracellular domain (ECD) that contains a stalk region and one C-type lectin domain (CTLD). Within the ECD, human CLEC9a shares 57% aa sequence identity with mouse and rat CLEC9a. Although the CTLD of CLEC9a structurally resembles that of other C-type lectins, it lacks the conserved residues that typically mediate calcium and carbohydrate binding. CLEC9a is expressed as a disulfide-linked homodimer of approximately 50kD N-glycosylated subunits. Human CLEC9a expression is restricted to a subpopulation of BDCA-3+ conventional dendritic cells (cDC) and CD16-monocytes. BDCA-3+ cDC are analagous to mouse CD8+ cDC which are specialized in antigenic cross-presentation in complex with MHC class I molecules. In mouse, CLEC9a is additionally expressed on plasmacytoid dendritic cells. CLEC9a ligation enhances antigen uptake and processing, leading to presentation on MHC class I and cytotoxic T cell (CTL) priming. In mouse, CLEC9a recognizes normally intracellular determinant(s) of necrotic cells and mediates their uptake by the dendritic cell. The subsequent antigenic cross-presentation to CTL is important for clearing necrotic cellular debris. CLEC9a signaling triggers activation of the tyrosine kinase Syk. \n\nSource: \nNS0-derived, murine myeloma cell line human CLEC9a (Lys57-Val241).\n\nSDS-PAGE:\n55-65kD, reducing conditions\n\nActivity:\nMeasured by flow cytometry for its ability to bind a ligand expressed in FasL-treated mouse B cells. At 100ng/ml, (same/more than)50% of 7AAD positive cells bind to rhCLEC9a.\n\nEndotoxin Level: (same/less than)1EU/1ug (LAL)\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C5839-73K

Size

50ug

Form

Supplied as a lyophilized powder from PBS. Reconstitute with sterile PBS.

Purity

~95% (SDS-PAGE, reducing conditions)

References

1. Huysamen, C. and G.D. Brown (2009) FEMS Microbiol. Lett. 290:121. 2. Geijtenbeek, T.B.H. et al. (2004) Annu. Rev. Immunol. 22:33. 3. Huysamen, C. et al. (2008) J. Biol. Chem. 283:16693. 4. Caminschi, I. et al. (2008) Blood 112:3264. 5. Sancho, D. et al. (2008) J. Clin. Invest. 118:2098. 6. Dudziak, D. et al. (2007) Science 315:107. 7. Sancho, D. et al. (2009) Nature 458:899.