CLED Agar (Cystine-Lactose-Electrolyte-Deficient) media was first formulated by Sandys and later modified by Mackey and Sandys. This medium supports the growth of urinary pathogens and gives good colonial differentiation of these bacteria. Lactose is the carbon source. Bromthymol blue is the indicator dye. bromthymol blue, Organisms that are lactose fermenting produce yellow colonies while non-lactose fermenting bacteria give blue colonies. Since there are no salts
(electrolyte-deficient) in this media the swarming of Proteus sp. is suppressed. CLED Agar is recommended as a dip inoculum transport medium for urine specimens. A quantitative count method for urine cultures called the Drop Plate method was devised by Neblett.
Appearance:
Light beige, homogeneous, free flowing powder.
Solubility:
Light greyish-green, clear, complete.
pH (25 degrees C):
7.3
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