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Clostridium botulinum A Toxoid

Cat no: C5851-40B

Clostridium botulinum A Toxoid

Applications: \nSuitable for use in ELISA, RIA, Immunofluorescence and Inhibition of BoTox-A binding to Neuro2A Cells. Other applications not tested.\n\nRecommended Dilutions:\nImmunofluorescence: 1:50; stains Neuro2A cells pre-treated with GT1b ganglioside using indirect immunocytochemical staining procedure. Following treatment, cells were washed 4 times, fixed in a neutral buffered formalin for 10 minutes, permeablized and blocked. Reacted for 60 minutes with C5851-40B and then an IgG (FITC) goat anti-mouse. The staining pattern was observed using epifluorescent microscopy.\nInhibition: Inhibits the binding of BoTox-A to Neuro2A cells treated with GT1b ganglioside prior to the addition of the toxin, and prior incubation of the antibody with the synthetic peptide. Tne immunogen has been shown to eliminate the inhibition of cellular binding by the antibody.\nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies: \nI1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab GtxMo\nI1904-23: IgG (FITC) (X-Adsorbed) Pab GtxMo\nI1904-04A: IgG, H&L (FITC) Pab GtxMo\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap

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SPECIFICATIONS

Catalog Number

C5851-40B

Size

1ml

Applications

ELISA, IF

Hosts

Mouse

Form

Supplied as a liquid.

P Type

Mab

Purity

Supernatant

Isotype

IgG

Additional Info

Recognizes BoTox-A (1280-1292) and whole BoTox-A. Does not react with BoTox-B, BoTox-C, BoTox-E or BoTox-F (1272-1280).

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