C. botulinum is a large anaerobic bacillus that is widely distributed wherever there is organic material (e.g. soil, sediments of lakes and ponds, decaying vegetation). Seven toxigenic types of the organism exist, producing immunologically distinct forms of botulinum toxin (A, B, C1, D, E, F, and G). A multivalent toxoid (a toxin modified to remove the toxic properties but maintain the ability to stimulate an immune reaction) evokes good protective antibiody response.
Applications:
Suitable for use in Dot Blot, ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 1:10,000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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