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Clostridium botulinum Toxin E (aa2-17)

Clostridium botulinum Toxin E (aa2-17)

Cat no: C5851-82J

Supplier: United States Biological
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\Clostridium botulinum E Toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase. It has a non-toxic component which is necessary to maintain toxicity. Applications: Suitable for use in ELISA, Immunofluorescence, Immunocytochemistry and RIA. Other applications have not been tested. Recommended Dilutions: Indirect Immunocytochemistry: 1:50 Note: Prior incubation of the antibody with the synthetic peptide immunogen (cat #C5851-82J1) has been shown to eliminate the inhibition of cellular binding by the antibody. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C5851-82J
Hosts: Mouse
Applications: ELISA, Immunocytochemistry, Immunofluorescence
Size: 500ul
Form: Supplied as a liquid. No preservatives added.
P type: Mab
Isotype: IgG
Purity: Supernatant
Additional info: Recognizes whole Clostridium botulinum Toxin E, aa2-17. Stains Neuro2A cells treated with GT1b ganglioside prior to the addition of C. botulinum Toxin E. Inhibits the entry of C. botulinum Toxin E to Neuro2A cells treated with GT1b ganglioside prior to the addition of the toxin. Does not crossreact with C. botulinum Toxin A, B, C, or F (aa1-16).

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