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Clostridium botulinum Toxin E (aa2-17)

Cat no: C5851-82K


Supplier: United States Biological
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Applications: Suitable for use in ELISA, Immunofluorescence, Indirect Immunocytochemistry and RIA. Other applications have not been tested. Recommended Dilutions: Indirect Immunocytochemistry: Prior incubation of the antibody with synthetic peptide immunogen (C5851-82J1) has been shown to eliminate the inhibition of cellular binding by the antibody. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C5851-82K
Hosts: Mouse
Applications: ELISA, Immunocytochemistry, Immunofluorescence
Size: 500ul
Form: Supplied as a liquid. No preservatives added.
P type: Mab
Isotype: IgM
Purity: Supernatant
Additional info: Binds to intact whole C. botulinum Toxin E, amino acids 2-17. Has been found to stain Neuro2A cells treated with GT1b ganglioside prior to the addition of C. botulinum Toxin E. Inhibits the entry of C. botulinum Toxin E to Neuro2A cells treated with GT1b ganglioside prior to the addition of the toxin.Cross-reactivity: C. botulinum Toxin E (2-17) and C. botulinum Toxin E: 100%, C. botulinum Toxin A, B, C and F (1-16): 0%

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