Calponins are abundant proteins in smooth muscle and are the products of three distinct genes. The most abundant form is alpha-calponin (h1 or basic) consisting of 292aa (34kD) and b-calponin, which is 252aa in length. Two other genes encode neutral calponin (h2 calponin) and acidic calponin are expressed at lower levels in smooth and non-muscle cells. Other more widely expressed calponin members include SM22, and transgelin. It is an abundant heat stable, basic protein in smooth muscle where it is present as a 34kD species. In other tissues a more acidic isoform (acidic CaP) is present. It is calcium independent but binds calmodulin in a sensitive manner. It is also capable of binding to actin, troponin C and tropomyosin. The tissue-specific h1 variant is involved in the regulation of the contraction/relaxation cycle in smooth muscle, probably by blocking the weak S1 binding site for myosin on actin, and plays a key role in stabilizing the structural integrity of blood vessels.
Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry (Formalin fixed paraffin embedded): 3-6ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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