CNOT4 (CCR4-NOT Transcription Complex Subunit 4, CCR4-associated Factor 4, E3 Ubiquitin-protein Ligase CNOT4, Potential Transcriptional Repressor NOT4Hp, NOT4)

Cat no: C2099-68W1

Supplier: United States Biological

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CNOT4 is a 575aa protein with E3 ubiquitin ligase activity, mainly involved in protein modification and protein ubiquitination. It contains a ring type zinc finger domain of the C4C4 type, an RNA recognition motif and a bipartite nuclear localization signal. Studies show that CNOT4 is a component of the CCR4-NOT (CAF1) core complex that functions as general transcription regulation complex and has both a positive as well as a negative effect on the transcription of multiple functionally unrelated genes, thus controls gene expression. CNOT4 interacts with CNOT1 via C-terminus and with E2 ubiquitin ligases via C4C4 RING domain. Reports suggest that CNOT4 gets phosphorylated in response to DNA damage on consensus sites recognized by ATM and ATR. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.05-0.1ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Human skeletal muscle lysate Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Catalogue number:	C2099-68W1
Reactivities:	Human, Mouse, Rat, Bovine, Chicken/Bird, Xenopus/Amphibian
Hosts:	Rabbit
Applications:	Western Blot
Size:	100ug
Form:	Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
P type:	Pab
Isotype:	IgG
Purity:	Purified by immunoaffinity chromatography.
References:	1. Albert, T.K. et al. Nucleic Acids Res. 28:809-817 (2000). 2. Matsuoka, S. et al. Science. 316:1160-1166 (2007). 3. Albert, T.K. et al. EMBO J. 21:355-364 (2002).
Additional info:	Recognizes human CNOT4. Species Crossreactivity: chicken, bovine, mouse, rat, xenopus. Species sequence homology: pufferfish.

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CNOT4 (CCR4-NOT Transcription Complex Subunit 4, CCR4-associated Factor 4, E3 Ubiquitin-protein Ligase CNOT4, Potential Transcriptional Repressor NOT4Hp, NOT4)

Cat no: C2099-68W1

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