Coagulation Factor VII Inhibitor Plasma, Moderate (FIP)

Supplier:

United States Biological

Cat no: C7465-07A

Coagulation Factor VII Inhibitor Plasma, Moderate (FIP)

Coagulation Factor Inhibitor Plasmas (FIPs) are produced from normal human plasmas from which specific factors have been removed by selective affinity immuno-adsorption and an antibody inhibitory to the specific factor is added to provide neutralizing activity. These products can be used as a substitute for plasmas from patients that have developed neutralizing antibodies to coagulation factors. All of these products are intended for research use only.\n\nActivity:\nFIPs are produced to one or more inhibitor activity categories:\nпїЅMild: 1-10 BU/ml\nпїЅModerate: (same/more than) 10-50 BU/ml\nпїЅStrong: (same/more than) 50-200 BU/ml\nThe inhibitor potency is measured by standard Bethesda activity assay.\n\nAssay Results:\nPT (HemosIL PT-Fib): 46.6 sec\nAPTT (HemosIL APTT-SP): 34.6 sec\nFibrinogen (clottable): 2.94g/L\nFVII (activity): < 0.01U/ml\nFVII inhibitor activity (Bethesda assay): 27BU/ml\n\nBethesda assay performed at a 1:20 dilution, then mixed with an equal volume of normal plasma and incubated at 37C for 120 minutes. The FVII activity was measured by one stage clotting assay and the resiual FVII activity was calculated as a percentage activity compared to a buffer control run in parallel. The residual FVII activity is converted to Bethesda units using the Bethesda chart or graph. The Bethesda value derived (BU/ml) is multiplied by the initial dilution of the sample to obtain the corrected inhibitor concentration.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C7465-07A

Size

1ml

Form

Supplied as a liquid in 20mM HEPES.

Purity

Purified by immunoaffinity chromatography.

Read more on Supplier website

SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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