Cofilin

Cat no: C7506-10C

Supplier: United States Biological

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Cofilin belongs to the actin-binding ADF protein family. It contains one ADF-H domain. Cofilin controls reversible actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin at a 1:1 ratio. It is the major component of both intranuclear and cytoplasmic actin rods. It is involved in the translocation of the actin-cofilin complex from the cytoplasm to the nucleus. This protein is widely distributed in various tissues. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.05-0.2ug/ml Western Blot: 0.5-1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute by adding sterile 40-50% glycerol, ddH2. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Catalogue number:	C7506-10C
Reactivities:	Human, Mouse
Hosts:	Rabbit
Applications:	ELISA, Western Blot
Size:	40ug
Form:	Supplied as a lypholized powder from PBS, pH 7.4, 0.02% sodium azide.
P type:	Pab
Isotype:	IgG
Purity:	Purified by immunoaffinity chromatography.
Additional info:	Recognizes endogenous levels of human cofilin protein. Species Crossreactivity: mouse. Species sequence homology: Cynomolgus monkey, sheep, porcine, and rat cofilin protein.

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Cofilin

Cat no: C7506-10C

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