Cofilin belongs to the actin-binding protein ADF family and it contains 1 ADF-H domain. Cofilin controls reversible actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin at a 1:1 ratio. It is the major component of intranuclear and cytoplasmic actin rods. It is involved in the translocation of the actin-cofilin complex from cytoplasm to nucleus. This protein is widely distributed in various tissues.
Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
ELISA: 0.05-0.2ug/ml
Western Blot: 0.5-1ug/ml
Immunohistochemistry: 5-10ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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