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Cofilin

Cofilin

Cat no: C7506-15A

Supplier: United States Biological
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Cofilin is a small phosphoinositide-sensitive, actin-binding protein capable of depolymerizing actin filaments in vitro. Under certain conditions, it fragments the filaments and accelerates actin subunit dissociation from their Applications: Suitable for use in Immunofluorescence and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000. Detects a band at approximately 19kDa. Use whole extracts of human A-431 epidermoid carcinoma, rat PC-12 pheochromocytoma, and dog MDCK kidney cells, or purified recombinant chicken muscle cofilin. Immunofluorescence: 1:1000 using NIH-NT3 fibroblasts Optimal dilutions to be determined by the researcher. Cellular Localization: Cytoplasmic and Nuclear Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C7506-15A
Reactivities: Human, Mouse, Rat, Canine, Chicken/Bird, Porcine
Hosts: Rabbit
Applications: Immunofluorescence, Western Blot
Size: 50ul
Form: Supplied as a liquid in PBS, pH 7.4, containing sodium azide
P type: Pab
Isotype: IgG
Purity: Purified
Alternative names: 18kD phosphoprotein, CFL1, Cofilin 1 non muscle, Cofilin non muscle isoform, p18
References: 1. Nishida, E., et al., Biochemistry, 23, 5307-5316 (1984). 2. Nishida, E., et al., Proc. Natl. Acad. Sci. (USA), 84, 5262-5266 (1987). 3. Moriyama, K., and Yahara, I., EMBO J., 18, 6752-6761 (1999). 4. Bamburg, R., Annu. Rev. Cell. Dev. Biol., 15, 185-230 (1999). 5. Carlier, M.F., et al., J. Cell Biol., 136, 1307-1322 (1997). 6. Maciver, S.K., et al., J. Cell. Biol., 278, 1611-1620 (1991). 7. Ohta, Y., et al., J. Biol. Chem., 264, 16143-16148 (1989). 8. Suzuki, K.T., et al., J. Biol. Chem., 270, 19551-19556 (1997). 9. Minamide, A., et al., Nature Cell Biol., 2, 628-636 (2000). 10. Arber, S., et al., Nature, 393, 805-809 (1998).
Additional info: Recognizes human Cofilin. Species Crossreactivity: mouse, rat, chicken, canine and porcine.

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