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Cofilin, phosphorylated (Ser3) (Cofilin-1, 18kD Phosphoprotein, p18, Cofilin, Non-muscle Isoform, CFL1, CFL)

Cofilin, phosphorylated (Ser3) (Cofilin-1, 18kD Phosphoprotein, p18, Cofilin, Non-muscle Isoform, CFL1, CFL)

Cat no: C7506-25E

Supplier: United States Biological
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Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:500-1:1000 Immunohistochemistry:1:50-1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C7506-25E
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. Kobayashi M., et al. (2006) EMBO J 25(4):713-26. 2. Wany Y, et al. (2005) Biol Chem 280(13):12683-9. 3. Smith-Beckerman DM., et al. (2005) Mol Cell Proteomics 156-68.
Additional info: Recognizes endogenous levels of human Cofilin only when phosphorylated at serine 3. Species Crossreactivity: mouse, rat

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