COL1A1 (OI4, Alpha-1 type I collagen, COL1A1/PDGFB, OI/EDS)

Collagen, type I, alpha 1, also known as COL1A1, is a human gene that encodes the major component of type I collagen, the fibrillar collagen found in most connective tissues, including cartilage. This gene is mapped to 17q21.33. This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. \n\nUniProt Number:\nP11087\n\nGene ID:\nCOL1A1\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry (Paraffin and Frozen), and Immunocytochemistry.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Handling:\nStore at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term.\nAvoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

144375

Size

100ug

Applications

ICC, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Aihara, M., Lindsey, J. D., Weinreb, R. N. Ocular hypertension in mice with a targeted type I collagen mutation. Invest. Ophthal. Vis. Sci. 44: 1581-1585, 2003.\n2. Cabral, W. A., Makareeva, E., Colige, A., Letocha, A. D., Ty, J. M., Yeowell, H. N., Pals, G., Leikin, S., Marini, J. C. Mutations near amino end of alpha-1(I) collagen cause combined osteogenesis imperfecta/Ehlers-Danlos syndrome by interference with N-propeptide processing. J. Biol. Chem. 280: 19259-19269, 2005.\n

Additional Info

Recognizes human, mouse and rat COL1A1. No crossreactivity with other proteins.