Colcemid (Demecolcine, BRN 2822892)

Anti-cancer compound. Cell synchronization agent. Used as a drug in chemotherapy by synchronizing tumor cells at metaphase, the radiosensitive stage of the cell cycle. Microtubule assembly inhibitor. Depolymerizes microtubules and limits microtubule formation (inactivates spindle fibre formation). Inhibits mitosis during cell division at metaphase by inhibiting spindle formation. Low concentrations affect microtubule dynamics and cell migration, while high concentrations promote microtubule detachment from microtubule organizing center. Apoptosis inducer. Thrombopoietic agent.\n\nStorage and Stability:\nShort-term Storage: +4 degrees C\nLong-term Storage: -20 degrees C\nStable for at least 2 years after receipt when stored at -20 degrees C.

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SPECIFICATIONS

Catalog Number

044675

Size

1mg

Purity

>98% (HPLC)

References

Product Reference: \n?Effect of colcemid on fibroblast motility: M.H. Gail & C.W. Boone; Exp. Cell Res. 65, 221 (1971)\n?Mitosis in human leukemic leukocytes during colcemid inhibition and recovery: M. McGill & B. R. Brinkley; Cancer Res. 32, 746 (1972)\n?The effects of colcemid on hematopoiesis in the mouse: P.A. Bunn Jr., et al.; J. Clin. Invest. 58, 1280 (1976)\n?Colcemid and colchicine binding to tubulin. Similarity and dissimilarity: A.C. Banerjee & B. Bhattacharyya; FEBS Lett. 99, 333 (1979)\n?Comparison of mitostatic effect, cell uptake and tubulin-binding activity on colchicine and colcemid: A.S. Serpinskaya, et al.; Biochim. Biophys. Acta 673, 86 (1981)\n?Action of demecolcine (colcemid) in the murine sarcoma 180 tumor: T. Nomura & J.E. Plager; Cancer Treat. Rep. 65, 283 (1981)\n?Colcemid effects on B16 melanoma cell progression and aberrant mitotic division: B.K. Bhuyan, et al.; J. Cell Physiol. 132, 237 (1987)\n?Colcemid and the mitotic cycle: C.L. Rieder & R.E. Palazzo; J. Cell. Sci. 102, 387 (1992)\n?Induction of apoptosis by the anti-tubulin drug colcemid: relationship of mitotic checkpoint control to the induction of apoptosis in HeLa S3 cells: S.W. Sherwood, et al.; Exp. Cell. Res. 215, 373 (1994) \n?Effects of colcemid concentration on chromosome aberration analysis in human lymphocytes: R. Kanda, et al.; J. Radiat. Res. (Tokyo) 35, 41 (1994)\n?Cell cycle arrest by Colcemid differs in human normal and tumor cells: M.N. Jha, et al.; Cancer Res. 54, 5011 (1994) \n?Dissociation of nuclear and cytoplasmic cell cycle progression by drugs employed in cell synchronization: L. Urbani, et al.; Exp. Cell. Res. 219, 159 (1995)\n?Expression of apoptosis and cell cycle related genes in proliferating and colcemid arrested cells of divergent lineage: B.W. Gallaher, et al.; Cell. Mol. Biol. 46, 79 (2000)\n?Inhibition of Cell Migration and Cell Division Correlates with Distinct Effects of Microtubule Inhibiting Drugs: H. Yang, et al.; J. Biol. Chem. 285, 32242 (2010)\n?

Alternative Names

Demecolcine, BRN 2822892