Home  >  Products  >  Collagen III (Collagen alpha-1(III) Chain, Collagen III alpha 1 Chain Precursor, Collagen III alpha 1 Polypeptide, Collagen Type III alpha 1, COL3A1, Ehlers-Danlos Syndrome Type IV Autosomal Dominant, EDS4A, Fetal Collagen, FLJ34534, Type III Collagen)

Collagen III (Collagen alpha-1(III) Chain, Collagen III alpha 1 Chain Precursor, Collagen III alpha 1 Polypeptide, Collagen Type III alpha 1, COL3A1, Ehlers-Danlos Syndrome Type IV Autosomal Dominant, EDS4A, Fetal Collagen, FLJ34534, Type III Collagen)

Cat no: C7510-44M


Supplier: United States Biological
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Collagens are highly conserved across species and are vital in the formation of connective tissues. Collagen III is found in most soft connective tissues along with Collagen I. Defects in collagen III are associated with Ehlers-Danlos syndrome type 3 and type 4 and with aortic aneurysm abdominal (AAA). Applications: Suitable for use in ELISA, Immunohistochemistry, Immunocytochemistry and Dot Blot. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Frozen): 1:20 if Peroxidasae labeled secondary is used. If FITC is used dilute 1:10. Not tested on paraffin. ELISA: Native human collagen III Dot Blot: Native human collagen III Optimal dilutions to be determined by the researcher. Immunohistochemistry Positive Control: Human kidney, liver, skin, heart Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C7510-44M
Reactivities: Human
Hosts: Rabbit
Applications: ELISA, Immunocytochemistry, Immunohistochemistry
Size: 1ml
Form: Supplied as a lyophilized powder. Reconstitute with 1ml sterile ddH2O.
P type: Pab
Isotype: IgG
Purity: Purified by affinity chromatography.
References: 1. Amenta, et al., 1986, Collagen rel. res. 6, 125-152. 2. Rukosuev, et al., 1990, Histochem. 89, 11-16. 3. Shekhonin, et al., 1985, Collagen rel. res. 5, 355-368.
Additional info: Recognizes human Collagen III. Minimal crossreactions with other collagens were only found if 20-50 times higher concentrations were used of other collagens.

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