Collagen Type I (Biotin)

This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.\n\nApplications: \nSuitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:5000-1:50,000\nIndirect ELISA: 1:4000-1:8000\nWestern Blot: 1:5000-1:50,000\nImmunohistochemistry (frozen): 1:50-1:200\nImmunoprecipitation: 1:5000-1:10,000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C7510-10X

Size

50ug

Applications

ELISA, IF, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Bov

Conjugates

Biotin

Form

Supplied as a liquid in PBS, pH 7.6, 15mg/ml BSA, 0.01% sodium azide. Labeled with Biotin.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Stefanovic, B, Schnabl, B, Brenner, DA (2002) Inhibition of collagen alpha 1(I) expression by the 5#039; stem-loop as a molecular decoy. J.Biol. Chem. 277(20):18229-18237.

Additional Info

Recognzies human Collagen Type I. Does not crossreact with Type II, III, IV, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible. Species Crossreactivity: bovine. Species Sequence Homology: mammals.

Alternative Names

COL1A1, Collagen Of Skin Tendon And Bone, OI4, Osteogenesis Imperfecta Type IV, Pro alpha 1(I) collagen