Collagen Type I (Biotin)

Cat no: C7510-10X

Supplier: United States Biological

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This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins. Applications: Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:5000-1:50,000 Indirect ELISA: 1:4000-1:8000 Western Blot: 1:5000-1:50,000 Immunohistochemistry (frozen): 1:50-1:200 Immunoprecipitation: 1:5000-1:10,000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Catalogue number:	C7510-10X
Reactivities:	Human, Bovine
Hosts:	Rabbit
Applications:	ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugates:	Biotin
Size:	50ug
Form:	Supplied as a liquid in PBS, pH 7.6, 15mg/ml BSA, 0.01% sodium azide. Labeled with Biotin.
P type:	Pab
Isotype:	IgG
Purity:	Purified by immunoaffinity chromatography.
Alternative names:	COL1A1, Collagen Of Skin Tendon And Bone, OI4, Osteogenesis Imperfecta Type IV, Pro alpha 1(I) collagen
References:	1. Stefanovic, B, Schnabl, B, Brenner, DA (2002) Inhibition of collagen alpha 1(I) expression by the 5#039; stem-loop as a molecular decoy. J.Biol. Chem. 277(20):18229-18237.
Additional info:	Recognzies human Collagen Type I. Does not crossreact with Type II, III, IV, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible. Species Crossreactivity: bovine. Species Sequence Homology: mammals.

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Collagen Type I (Biotin)

Cat no: C7510-10X

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