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Collagen Type II (Alpha-1 Type II Collagen, Collagen alpha-1(II) Chain Precursor, Collagen Type II alpha 1 Polypeptide, COL2A1, ANFH, AOM, Cartilage Collagen, Chondrocalcin, COL11A3, MGC131516, SEDC)

Collagen Type II (Alpha-1 Type II Collagen, Collagen alpha-1(II) Chain Precursor, Collagen Type II alpha 1 Polypeptide, COL2A1, ANFH, AOM, Cartilage Collagen, Chondrocalcin, COL11A3, MGC131516, SEDC)

Cat no: C7510-37C

Supplier: United States Biological
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Applications: Suitable for use in Immunohistochemistry, ELISA and Dot Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry (Frozen): 1:20 if Peroxidasae labeled secondary is used. If FITC is used dilute 1:10. Not tested on paraffin. ELISA: Native human collagen II Dot Blot: Native human collagen II Optimal dilutions to be determined by the researcher. IHC Positive Control: Human kidney, liver, skin, heart Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with 1ml ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C7510-37C
Reactivities: Human
Hosts: Rabbit
Applications: ELISA, Immunohistochemistry
Size: 1ml
Form: Supplied as a lyophilized powder. Reconstitute with 1ml sterile ddH2O.
P type: Pab
Purity: Purified by ammonium sulfate precipitation and DEAE chromatography.
References: 1. Amenta, et al., 1986, Collagen rel. res. 6, 125-152. 2. Rukosuev, et al., 1990, Histochem. 89, 11-16. 3. Shekhonin, et al., 1985, Collagen rel. res. 5, 355-368.
Additional info: Recognizes human Collagen II. Minimal crossreactions with other collagens were only found if 5-10 times higher concentrations were used of other collagens. Does not react with fibronectin, fibrinogen or laminin.

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