Applications:
Suitable for use in Immunohistochemistry, Immunocytochemistry, ELISA and Dot Blot. Other applications not tested.
Recommended Dilution:
Immunohistochemistry (Frozen): 1:20 if Peroxidasae labeled secondary is used. If FITC is used dilute 1:10. Not tested on paraffin.
ELISA: Native human collagen II
Dot Blot: Native human collagen II
Optimal dilutions to be determined by the researcher.
IHC Positive Control:
Human kidney, liver, skin, heart
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with 1ml ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.