Home  >  Products  >  Collagen Type II (Alpha-1 Type II Collagen, Collagen alpha-1(II) Chain Precursor, Collagen Type II alpha 1 Polypeptide, COL2A1, COL2A1 Protein, ANFH, AOM, Cartilage Collagen, Chondrocalcin, COL11A3, MGC131516, SEDC)
Collagen Type II (Alpha-1 Type II Collagen, Collagen alpha-1(II) Chain Precursor, Collagen Type II alpha 1 Polypeptide, COL2A1, COL2A1 Protein, ANFH, AOM, Cartilage Collagen, Chondrocalcin, COL11A3, MGC131516, SEDC)

Collagen Type II (Alpha-1 Type II Collagen, Collagen alpha-1(II) Chain Precursor, Collagen Type II alpha 1 Polypeptide, COL2A1, COL2A1 Protein, ANFH, AOM, Cartilage Collagen, Chondrocalcin, COL11A3, MGC131516, SEDC)

Cat no: C7510-20R

Supplier: United States Biological
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Applications: Suitable for use in ELISA, Immunofluorescence, and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:500 Western Blot: 1:1000-1:2000 Immunofluorescence: Neat-1:5 Suitable for use on fresh, frozen or acetone fixed material. Mild pepsin digestion is recommended to enhance staining. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C7510-20R
Reactivities: Human, Mouse, Rat, Bovine, Canine, Porcine, Sheep
Hosts: Mouse
Applications: ELISA, Immunofluorescence, Western Blot
Size: 100ul
Form: Supplied as a liquid. No preservatives added.
P type: Mab
Isotype: IgG1
Purity: Ascites
Alternative names: CB11, Collagen II alpha 1 (Primary Osteoarthritis Spondyloepiphyseal Dysplasia Congenital)
References: 1. Wilkins, B. S. & Jones, D. B. (1995) Immunohistochemical characterization of intact stromal layers in long-term cultures of human bone marrow Br. J. Haematol. 90: 757 - 7662. Miller, E. J. (1972) Structural studies on cartilage collagen employing limited cleavage and solubilization with pepsin Biochemistry 11, p.4903-49093. Glant, T. T. et al. (1985) Appearance and persistence of fibronectin in cartilage. Specific interaction of fibronectin with collagen type II Histochemistry 82: 149 - 158.
Additional info: Recognizes human collagen type II, the main collagen in cartilaginous tissue. This antibody reacts with both pepsin solubilised and CNBr-cleaved human and bovine collagen type II. No crossreactivity is seen with types I, III, V or IX., Species Crossreactivity: rat, bovine, sheep, mouse, canine, porcine.

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