Collagen Type II (BSA & Azide Free)

Applications: \nSuitable for use in Immunofluorescence, ELISA and Western Blot. Not suitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml for 2 hours at RT.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nCartilage\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C7510-19X

Size

100ug

Applications

ELISA, IF, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Ch/Bird

Form

Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See C7510-19.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a,k

References

1. Mayne, R., et al., Connective Tissue Research 31(1): 11-21 (1994). 2. Chen, Q., et al., Developmental Dynamics 196(1): 47-53 (1993).

Additional Info

Recognizes chicken Collagen Type II. Show no crossreactivity with Types I, III, IV, V, VI, IX, X or XI. In Western Blot, reacts with the TCA fragment of lathyritic Type II Collagen after digestion with mammalian collagenase. It does not react with pepsin-digested Type II Collagen. The epitope recognized is GGFDEK, slightly preceding the lysine residue involved in crosslinking. Two terminal glycine residues constitute a key structural element of the epitope. The epitope is lost after digestion of lathyritic Type II Collagen with recombinant Stromelysin. Species Crossreactivity: Human and mouse.