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Collagen Type III (Biotin)

Collagen Type III (Biotin)

Cat no: C7510-39U

Supplier: United States Biological
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C7510-39U is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:10000-1:50000 Western Blot: 1:1000-1:5000 Immunohistochemistry (Paraffin): 1:200-1:1000 Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: C7510-39U
Reactivities: Human, Bovine
Hosts: Rabbit
Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Conjugates: Biotin
Size: 100ug
Form: Supplied as a liquid in PBS, pH 7.6, 15mg/ml BSA, 0.01% thimerosal, 0.05% sodium azide. Labeled with Biotin.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
Alternative names: COL3A1, Collagen III alpha 1 chain precursor, Collagen III alpha 1 polypeptide, Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant), EDS4A, Fetal collagen, Type III collagen
References: 1. Sales, V. L., G. C. Engelmayr, Jr., et al. (2007). Protein Precoating of Elastomeric Tissue-Engineering Scaffolds Increased Cellularity, Enhanced Extracellular Matrix Protein Production, and Differentially Regulated the Phenotypes of Circulating Endothelial Progenitor Cells. 116: I-55-63.
Additional info: Recognizes human Collagen Type III. Negligible crossreactivity with Type I, II, IV, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible. Species Crossreactivity: bovine. Species Sequence Homology: mammals

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