Collagen Type III

HMG1 and HMG2, chromatin non-histone high mobility group proteins 1 and 2, are 27 and 25kD members of a family of proteins containing multiple HMG-boxes, conserved domains of 80 amino acids which mediate DNA binding of many proteins. HMG box domains recognize DNA structure, such as prebent, supercoiled or four way junction DNA, and non- specific DNA sequences. Both HMG1 and HMG2 contain an N-terminal HMG box, a central HMG box, and an acidic carboxy terminus. The acidic tails of these proteins contain multiple serine residues which match the phosphorylation consensus sites of casein kinase II, and phosphorylation of this domain appears to be important for proper functioning of these proteins. HMG1/2 have been shown to facilitate the binding of various sequence-specific transcription factors to their respective DNA binding sites, such as steroid hormone receptors, p53, and Oct. HMG1/2 may serve as architectural factors that recognize and mediate DNA structural changes that accompany various events such as DNA repair, transcription and replication.\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunohistochemistry (Fr). Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C7510-39V

Size

100ul

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Hum, Rat

Form

Ascites fluid

P Type

Mab

Purity

Ascites

Isotype

IgG1

References

1. Sanes JR et al. Molecular heterogeneity of basal laminae: isoforms of laminin and collagen IV at the neuromuscular junction and elsewhere. J Cell Biol 111:1685-99 (1990). <PUBMED:2211832>\n2. Superti-Furga A et al. Molecular defects of type III procollagen in Ehlers-Danlos syndrome type IV. Hum Genet 82:104-8 (1989). <PUBMED:2722184>\n3. Mayne R Preparation and applications of monoclonal antibodies to different collagen types. Clin Biochem 21:111-5 (1988). <PUBMED:3292079>

Additional Info

The antibody specifically recognizes native and denatured collagen type III. It does not recognize collagen types I, II, IV, V, VI and X. Species Crossreactivity: Crossreacts with Human and Rat.

Alternative Names

COL3A1, Collagen III alpha 1 chain precursor, Collagen III alpha 1 polypeptide, Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant), EDS4A, Fetal collagen, Type III collagen