Collagen Type III

These antibodies are well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.\n\nApplications: \nSuitable for use in ELISA, Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications have not been tested.\n\nRecommended Dilution:\nELISA: 1:5000-1:50,000\nImmunohistochemistry: 1:50-1:200\nImmunohistochemistry(Paraffin): 1:20-1:200\nImmunoprecipitation: 1:100\nWestern Blot: 1:1000-1:10,000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C7510-39X

Size

100ug

Applications

ELISA, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Rat, Bov, Rabbit

Form

Supplied as a liquid in 0.125M sodium borate, 0.075M sodium chloride, 0.005M EDTA, 0.1% sodium azide, pH 8.0

P Type

Pab

Purity

Purified by immunoaffinity chromatography

Isotype

IgG

Additional Info

Recognizes human Collagen Type III. Species Crossreactivity: rat, bovine, rabbit, and most mammals. Typically <1% cross-reactivity against other types of collagens was detected by ELISA against purified standards. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. Has negligible cross-reactivity with Type I, II, IV, V or VI collagens. Non-specific cross-reaction with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

Alternative Names

COL3A1, Collagen III alpha 1 chain precursor, Collagen III alpha 1 polypeptide, Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant), EDS4A, Fetal collagen, Type III collagen