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Collagen Type III

Cat no: C7510-41C

Collagen Type III

Pooled antisera are passed over DEAE-cellulose to produce IgG-enriched fraction, which is further subjected to absorption with immobilized total human serum proteins in order to remove non-speci?c antibodies. Next, the antisera fraction is absorbed with immobilized collagen types I, II, IV and V to remove crossreactive antibodies to antigenic determinants common for various collagen types. The af?nity puri?ed antibody is obtained by binding to immobilized native human collagen type III (the antigen used for immunization), followed by elution with acidic buffer, neutralization, dialysis, dispensing and lyophilization. Speci?city was ascertained by competition ELISA. Complete inhibition is found if the antibody was preincubated with collagen type III. Inhibition by other types of collagens is observed only at 20-50 times higher concentration.\n\nReconstitute with 1.0ml distilled water and add preservative if preferred.\n\nApplications: This antibody can be used for immunohistochemistry on frozen cells and sections. Suitable for dot-blotting and ELISA on native human collagen type III. Use on paraf?n sections is not tested. May be diluted at least 1:20 for immunohistochemical procedures if Peroxidase labeled secondary antibodies are applied. If a FITC-labeled secondary antibody is used the antibody can be diluted 1:10.

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SPECIFICATIONS

Catalog Number

C7510-41C

Size

1ml

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Af?nity Puri?ed, Lyophilized. No preservative added.

P Type

Pab

Purity

Purified by ammonium sulfate precipitation and cross-absorption on immobilized other collagen types and af?nity chromatography.

Isotype

IgG

References

1. Amenta et al. Collagen Rel. Res. 6. 125

Additional Info

Recognizes Collagen Type III.

Read more on Supplier website

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info
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