Collagen Type III

Type III collagen is present in the walls of arteries, intestines and the uterus, where it strenghtens the connective tissue. Patients with defects in the type III collagen (ex. Ehlers-Danlos syndrome) are at risk of rupture of major arteries or the intestine. Can be used for detection of collagens by ELISA. Has been used successfully for immunohistology on frozen unfixed sections of human (3) and kangaroo (4) skin, and of new canine tissue associated with biomaterial implants (2,5). If fixation of tissue is required, acetone or ethanol is recommended.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:250\nImmunohistochemistry: Unfixed, acetone or ethanol frozen sections.\nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nNS1/AG4.1 myeloma cells with spleen cells from mice. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C7510-43H

Size

100ul

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Hum, Rat, Can

Form

Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide. before the addition of glycerol to 40%.

P Type

Mab

Purity

Purified by Protein A/G affinity chromatography.

Isotype

IgG1,k

References

1. Werkmeister JA, Ramshaw JAM (1991) Multiple antigenic determinants on type III collagen. Biochem J 274:895-898.\n2. Werkmeister JA, Peters DE, Ramshaw JAM (1989) Development of monoclonal antibodies to collagens for assessing host-implant interactions. J Biomed Mater Res 23(A3):273-283.\n3. Werkmeister JA, Ramshaw JAM (1989) Monoclonal antibodies to collagens for immunofluorescent examination of human skin. Acta Derm Venereol 69:399-402. \n4. Stephens LJ, Werkmeister JA, Tebb TA, Ramshaw JAM (1991) Identification of type III collagen from kangaroo skin. Das Leder 42:41-44.\n5. Werkmeister JA, Glattauer V, Tebb TA, Ramshaw JAM, Edwards GA, Robets G (1991) Structural stability of long-term implants of a collagen-based vascular prosthesis. J Long Term Eff Med Implants 1:107-119.

Additional Info

Recognizes human type III collagen. It has been shown to have no cross-ractivity with type I and V collagens by ELISA and Western Blot. There is no evidence for cross-reactivity with other connective tissue proteins (laminin, fibronectin, elastin). Species Crossreactivity: canine, rat and kangaroo type III collagens by ELISA and Western Blot. The antibody does not show reactivity with calf and sheep type III collagen by ELISA and Western Blot.