Collagen Type IV (Arresten, ASLN, ATS, CA54, Canstatin, COL4A1, COL4A2, Collagen Alpha 1(IV) Chain, Collagen Alpha 2(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen IV Alpha 2 Polypeptide, Collagen of Basement Membrane Alpha 1 Chain, Collagen of Base

Collagen IV is a major constituent of the basement membranes along with laminins and enactins. It is composed of a1(IV) chain and a2(IV) chain in 2:1 ratio . It can form insoluble fibers with high tensile strength. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas. \n\nExcellent for the staining of routine formalin-fixed, paraffinembedded tissues.\n\nApplications:\nImmunohistology (Formalin/paraffin): (Use Ab at 1:50-1:100 for 30 min at RT) (Staining of formalin/paraffin tissues is enhanced by digestion of tissue sections with Protease XXV at 1mg/ml PBS for 5 minutes at 37 degrees C.\n\nPositive Control: Skin\n\nCellular Localization: Basement membrane\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degrees C or colder. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C7510-50D

Size

500ul

Applications

ELISA, IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid with 0.09% sodium azide.

P Type

Mab

Purity

Culture supernatant

Isotype

IgG1+IgG1

References

1. Odermatt B F, et. al. 91984) Proc Natl Acad Sci USA, 81:7343-7347.\n2. Lee CS; et al. Pathology, 1996, 28(2):135-8.\n3. Delaigue O; et al. Graefes Archive for Clinical and Experimental Ophthalmology, 1995 233: 331-8. and Experimental Ophthalmology, 1995 233: 331-8.\nAdditional Suggested References:\n1. Heikinheimo K; Salo T. Expression of basement membrane type IV collagen and type IV collagenases (MMP-2 and MMP-9) in human fetal teeth. Journal of Dental Research, 1995 May, 74(5):1226-34.\n2. Imamura T; Manabe T; Ohshio G; Wang ZH; Yamaki K; Yoshimura T; Suwa H; Imamura M. Immunohistochemical staining for type IV collagen and laminin in the stroma of human pancreatic cancer. International Journal of Pancreatology, 1995 Oct, 18(2):95-9.\n3. Razzaque MS; Cheng M; Horita Y; Nishihara M; Harada T; Taguchi T. Immunohistochemical analysis of type III and IV collagens in tubulointerstitial damage in human benign nephrosclerosis. Journal of International Medical Research, 1995 Nov-Dec, 23(6):480-6.\n4. Razzaque MS; Koji T; Horita Y; Nishihara M; Harada T; Nakane PK; Taguchi T. Synthesis of type III collagen and type IV collagen by tubular epithelial cells in diabetic nephropathy. Pathology, Research and Practice, 1995 Nov, 191(11):1099-104.\n5. Razzaque MS; Koji T; Kawano H; Harada T; Nakane PK; Taguchi T. Glomerular expression of type III and type IV collagens in benign nephrosclerosis: immunohistochemical and in situ hybridization study [published erratum appears in Pathol Res Pract 1995 Mar;191(2):179]. Pathology, Research and Practice, 1994 May, 190(5):493-9.\n6. Vega JA; Esteban I; Naves FJ; del Valle ME; Malinovsky L. Immunohistochemical localization of laminin and type IV collagen in human cutaneous sensory nerve formations. Anatomy and Embryology, 1995 Jan, 191(1):33-9.\n7. Berndt A; Kosmehl H; Katenkamp D; Tauchmann V. Appearance of the myofibroblastic phenotype in Dupuytren's disease is associated with a fibronectin, laminin, collagen type IV and tenascin extracellular matrix. Pathobiology, 1994, 62(2):55-8.\n8. Kumagai S; Kojima S; Imai K; Nakagawa K; Yamamoto E; Kawahara E; Nakanishi I. Immunohistologic distribution of basement membrane in oral squamous cell carcinoma. Head and Neck, 1994 Jan-Feb, 16(1):51-7.\n9. Nakagawa H; Yagihashi S. Expression of type IV collagen and its degrading enzymes in squamous cell carcinoma of lung. Japanese Journal of Cancer Research, 1994 Sep, 85(9):934-8.\n10. Terada T; Nakanuma Y. Expression of tenascin, type IV collagen and laminin during human intrahepatic bile duct development and in intrahepatic cholangiocarcinoma. Histopathology, 1994 Aug, 25(2):143-50.\n11. Zhao M; Matter K; Laissue J; Zimmermann A. Collagen type IV in human liver grafts: quantitative relationship to graft survival and rejection. Transplantation Proceedings, 1994, 26(4):2211-4.\n12. Zhu D; Kim Y; Steffes MW; Groppoli TJ; Butkowski RJ; Mauer SM. Glomerular distribution of type IV collagen in diabetes by high resolution quantitative immunochemistry. Kidney International, 1994 Feb, 45(2):425-33.\n13. Haramoto T; Makino H; Ikeda S; Ota Z. Ultrastructural localization of three major basement membrane components-- type IV collagen, heparan sulfate proteoglycan and laminin in the normal human GBM. Nippon Jinzo Gakkai Shi. Japanese Journal of Nephrology, 1993 Aug, 35(8):933-9.\n14. Hatae T; Ishibashi T; Yoshitomi F; Shibata Y. Immunocytochemistry of types I-IV collagen in human\nanterior subcapsular cataracts. Graefes Archive for Clinical and Experimental Ophthalmology, 1993 Oct, 231(10):586-90.\n15. Katoh R; Muramatsu A; Kawaoi A; Komiyama A; Suzuki K; Hemmi A; Katayama S. Alteration of the basement membrane in human thyroid diseases: an immunohistochemical study of type IV collagen, laminin and heparan sulphate proteoglycan. Virchows Archiv. a, Pathological Anatomy and Histopathology, 1993, 423(6):417-24.\n16. Lallemand AV; Ruocco SM; Gaillard DA. Expression and immunohistochemical localization of laminin and type IV collagen in developing human fetal tracheal glands. International Journal of Developmental Biology, 1993 Sep, 37(3):491-5.\n17. Marshall GE; Konstas AG; Lee WR. Collagens in the aged human macular sclera. Current Eye Research, 1993 Feb, 12(2):143-53.\n18. NG HK; Wong AT. Expression of epithelial and extracellular matrix protein markers in meningiomas.\nHistopathology, 1993 Feb, 22(2):113-25.\n19. Schultka R; Gopel C; Schuppan D; Schmidt T. Agedependent changes of the immunohistochemical distribution of various collagen types and structural glycoproteins in the human uterine tube. Acta Histochemica, 1993 Dec, 95(2):139-53.\n20. Tsutsumi M; Urashima S; Matsuda Y; Takase S; Takada A. Changes in type IV collagen content in livers of patients with alcoholic liver disease. Hepatology, 1993 May, 17(5):820-7.\n21. Rukosuev VS. Immunofluorescent localization of collagen types I, III, IV, V, fibronectin, laminin, entactin, and heparan sulphate proteoglycan in human immature placenta. Experientia, 1992 Mar 15, 48(3):285-7.\n22. Yoshida T; Adachi E; Matsubara O; Kino J; Asamatsu C; Sato Y; Nakajima K; Fukushima S; Hayashi T.\nSemiquantitative analysis of the effects of fixation and paraffin embedding on immunoreactivity of renal basement membranes to a monoclonal antibody against type IV collagen. Archives of Histol and Cytol, 1992, 55(5):497-502.\n23. Nanaev AK; Rukosuev VS; Shirinsky VP; Milovanov AP; Domogatsky SP; Duance VC; Bradbury FM; Yarrow P; Gardiner L; d'Lacey C; et al. Confocal and conventional immunofluorescent and immunogold electron microscopic localization of collagen types III and IV in human placenta. Placenta, 1991 Nov-Dec, 12(6):573-95.\n24. Sinha AA; Gleason DF; DeLeon OF; Wilson MJ; Limas C; Reddy PK; Furcht LT. Localization of type IV collagen in the basement membranes of human prostate and lymph nodes by immunoperoxidase and immunoalkaline phosphatase. Prostate, 1991, 18:93-104.

Additional Info

Highly specific to type IV collagen. In kidney, Ab reacts with glomerular and tubular basement membranes, parts of mesangial matrix and the Bowman