Collagen Type IV (Arresten, ASLN, ATS, CA54, Canstatin, COL4A1, COL4A2, Collagen Alpha 1(IV) Chain, Collagen Alpha 2(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen IV Alpha 2 Polypeptide, Collagen Of Basement Membrane Alpha 1 Chain, Collagen Of Base

Applications: \nSuitable for use in ELISA, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:4000-1:8000. Assayed against 1ug of Collagen IV in a standard sandwich ELISA using S7973-97A: Streptavidin (HRP) and A0030: ABTS Stable Liquid Substrate 1.46mM/Liter for HRPO for 30 minutes at RT.\nImmunohistochemistry (Frozen tissue): 1:50-1:200 \nWestern Blot: 1:5000-1:10,000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nCertificate of Origin:\nAll components used in the preparation of this product are derived from starting materals of North American origin. Collection was performed in USDA inspected facilities. All materilas have been inspected and certified to be free of disease. Suitable for export.

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SPECIFICATIONS

Catalog Number

C7510-52F

Size

100ug

Applications

ELISA, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum

Conjugates

Biotin

Form

Supplied as a liquid in 0.125M sodium borate, 0.075M sodium chloride, 0.005M EDTA, pH 8.0, 10mg/ml BSA, 0.01% sodium azide. Labeled with Biotin. Biotin/Protein Ratio: 10-20 BAC molecules per rabbit IgG molecule..

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes Collagen Type IV. Typically less than 1% crossreactivity against other types of collagens was detected by ELISA against puri?ed standards. Some class speci?c anti-collagens may be speci?c for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-?xed, paraf?n embedded tissues. Reacts with most mammalian Type IV collagens and has negligible crossreactivity with Type I, II, III, V or VI collagens. Non-speci?c crossreactivity with other human serum proteins or non-collagen extracellular matrix proteins is negligible.