Collagen Type IV (Arresten, ASLN, ATS, CA54, Canstatin, COL4A1, COL4A2, Collagen Alpha 1(IV) Chain, Collagen Alpha 2(IV) Chain, Collagen IV Alpha 1 Polypeptide, Collagen IV Alpha 2 Polypeptide, Collagen of Basement Membrane Alpha 1 Chain, Collagen of Base

Applications: \nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry: 1:40-1:60 for 60 min at RT (Frozen & formalin/paraffin)\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C or colder. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

C7510-54

Size

500ul

Applications

IHC

Hosts

Mouse

Reactivities

Hum, Bov

Form

Supplied as concentrated tissue culture supernatant in 15mM sodium azide.

P Type

Mab

Purity

Tissue culture supernatant

Isotype

IgG1

References

1. Lee CS; Redshaw A; Boag G. Abnormalities in type IV collagen immunoreactivity in human laryngeal cancers. Pathology, 1996, 28(2):135-8.\n2. Delaigue O; Arbeille B; Rossazza C; Lemesle M; Roingeard P. Quantitative analysis of immunogold labellings of collagen types I, III, IV and VI in healthy and pathological human corneas. Graefes Archive for Clinical and Experimental Ophthalmology, 1995 Jun, 233(6):331-8.\n3. Heikinheimo K; Salo T. Expression of basement membrane type IV collagen and type IV collagenases (MMP-2 and MMP-9) in human fetal teeth. Journal of Dental Research, 1995 May, 74(5):1226-34.\n4. Imamura T; Manabe T; Ohshio G; Wang ZH; Yamaki K; Yoshimura T; Suwa H; Imamura M. Immunohistochemical staining for type IV collagen and laminin in the stroma of human pancreatic cancer. International Journal of Pancreatology, 1995 Oct, 18(2):95-9.\n5. Razzaque MS; Cheng M; Horita Y; Nishihara M; Harada T; Taguchi T. Immunohistochemical analysis of type III and IV collagens in tubulointerstitial damage in human benign nephrosclerosis. Journal of International Medical Research, 1995 Nov-Dec, 23(6):480-6.\n6. Razzaque MS; Koji T; Horita Y; Nishihara M; Harada T; Nakane PK; Taguchi T. Synthesis of type III collagen and type IV collagen by tubular epithelial cells in diabetic nephropathy. Pathology, Research and Practice, 1995 Nov, 191(11):1099-104.\n7. Razzaque MS; Koji T; Kawano H; Harada T; Nakane PK; Taguchi T. Glomerular expression of type III and type IV collagens in benign nephrosclerosis: immunohistochemical and in situ hybridization study [published erratum appears in Pathol Res Pract 1995 Mar;191(2):179]. Pathology, Research and Practice, 1994 May, 190(5):493-9.\n8. Vega JA; Esteban I; Naves FJ; del Valle ME; Malinovsky L. Immunohistochemical localization of laminin and type IV collagen in human cutaneous sensory nerve formations. Anatomy and Embryology, 1995 Jan, 191(1):33-9.\n9. Berndt A; Kosmehl H; Katenkamp D; Tauchmann V. Appearance of the myofibroblastic phenotype in Dupuytren

Additional Info

Recognizes Type IV collagen. Species Reactivity: Human and bovine. Does not react with Rat, porcine or canine.