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Collagen Type V

Collagen Type V

Cat no: C7510-55B

Supplier: United States Biological
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This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins Cellular Localization: Extracellular matrix Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry (Fr). Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C7510-55B
Reactivities: Human
Hosts: Rabbit
Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 100ug
Form: 0.005% EDTA, 0.125M Sodium Borate, 0.075M Sodium Chloride. pH 8.0
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
Additional info: Negligible crossreactivity with Type I, II, III, IV or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible. Species Crossreactivity: Crossreacts with Human. Not yet tested in other species.

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