Collagen Type V (Collagen-V, Alpha 1 Type V Collagen, Collagen alpha-1(V) Chain, Collagen Type V alpha 1, Col5A1, Alpha 2 Type V Collagen, Collagen alpha-2(V) Chain, Collagen Type V alpha 2, Collagen V alpha 2 Polypeptide, Col5A2, Collagen alpha-3(V) Chai

Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. \n\nApplications:\nSuitable for use in ELISA and Immunohistochemistry. Other applications not tested. \n\nRecommended Dilution:\nImmunohistochemistry (paraffin): 1:20. Requires pre-treatment with 1mg/ml pepsin in 0.5M acetic acid for 2 hours at 37C and multiple buffer washes is recommended.\nImmunohistochemistry (Frozen):1:20\nELISA: 1:1500\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, 1% BSA, 0.09% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

C7510-56M

Size

500ul

Applications

ELISA, IHC

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS/BBS, 1% dextran, 1% mannitol. Reconstitute with 500ul ddH2O, 1% BSA, 0.09% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Evans, M. J. et al. (2002) Fibroblast growth factor-2 during postnatal development of the tracheal basement membrane Am J Physiol Lung Cell Mol Physiol 283: 1263-1270.

Additional Info

Recognizes human collagen type V 100%. Crossreactivity: human collagen type IV <5%, human collagen type I and III collagen <3%, human plasma proteins <0.5%.

Alternative Names

COL5A2 Protein, Collagen alpha-2(V) Chain Precursor, OTTHUMP00000064637