Collagen Type XXIII (COL23A1, alpha 1, Collagen alpha-1(XXIII) chain, DKFZp434K0621)

Collagen XXIII a1 (COL23A1) is an epithelial cell type 2 transmembrane glycoprotein with multiple collagen-like domains in its extracellular region. Intracellular furin-mediated cleavage of the molecule generates a 58kD shed fragment that forms disulfide-linked trimers. An alternately spliced isoform consists of nearly 300 amino acids of the membrane proximal extracellular domain (ECD), flanked by substituted sequences. COL23A1 is upregulated in \nmany transformed cell lines and serves as an indicator for tumor aggressiveness in prostate cancer. Within the ECD, human COL23A1 shares 89% aa sequence identity with mouse and rat COL23A1. \n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.5-1ug/ml. The detection limit for rhCOL23A1 is approximately 0.1ng/well. \nWestern Blot:1ug/ml. Tissue lysates were resolved by SDS-PAGE,transferred to an Immobilon-P membrane and \nimmunoblotted with 1.0ug/ml sheep anti-humanCOL23A1.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute by adding sterile 40-50% glycerol, PBS, 0.02% sodium azide. Aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C7510-94

Size

100ug

Applications

ELISA, WB

Hosts

Sheep

Reactivities

Hum

Form

Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with sterile PBS. If 0.5ml of PBS is used, the antibody concentration will be 0.2mg/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes human COL23A1 in direct ELISAs and Western blots. In these formats, this antibody shows less than 5% cross-reactivity with Recombinant, Human COL25A1.