Collagen XXV (Collagen 25 alpha 1 chain; also CLAC-P) is a neuronal, 65-75kD member of the Collagenous transmembrane (TM) subfamily of the Collagen protein family. It is synthesized as a 654 amino acid (aa) type II TM glycoprotein that contains a 33 aa cytoplasmic region (aa 1-33) and a 600 aa extracellular domain (ECD) (aa 55-654). The ECD contains seven Collagen-like domains. Furin cleavage between Arg112-Glu113 generates a 70kD soluble fragment that homotrimerizes and binds to early ABeta amyloid deposits. There are at least five multiple splice forms that impact the ECD. Amino acids 139-146 and the seventh Collagen-like domain (aa 571-630) are most often affected. Over aa 189-654, human COL25A1 is 95% aa identical to mouse COL25A1.
Applications:
Suitable for use in Direct ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
Direct ELISA: 0.5-1.0ug/ml detection limit for rhCOL25A1 is ~ 0.5ng/well.
Western Blot: 1.0ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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