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Collagen Types I, II, III, IV, V (Collagen Type 1, Collagen Type 2, Colllagen Type 3, Colllagen Type 4, Colllagen Type 5)

Collagen Types I, II, III, IV, V (Collagen Type 1, Collagen Type 2, Colllagen Type 3, Colllagen Type 4, Colllagen Type 5)

Cat no: C7510-18W

Supplier: United States Biological
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Applications: Suitable for use in Immunohistochemistry, ELISA and Dot Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry (Frozen): 1:200 if peroxidase-labeled secondary is used. Not tested on paraffin. ELISA: Native and denatured human collagens Dot Blot: Native and denatured human collagens Optimal dilutions to be determined by the researcher. IHC Positive Control: Human kidney, liver, skin, heart Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with 1ml ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: C7510-18W
Reactivities: Human
Hosts: Rabbit
Applications: ELISA, Immunohistochemistry
Size: 1ml
Form: Supplied as a lyophilized powder. Reconstitute with 1ml sterile ddH2O.
P type: Pab
Purity: Purified by ammonium sulfate precipitation and DEAE chromatography. Has been cross-adsorbed with immobilized human serum proteins.
References: 1. Amenta, et al, 1986, Collagen rel. res. 6, 125-152. 2. Rukosuev, et al, 1990, Histochem. 89, 11-16. 3. Shekhonin, et al, 1985, Collagen rel. res. 5, 355-368.
Additional info: Recognizes human collagen types I-V.

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